![]() ![]() Received: OctoAccepted: FebruPublished: February 22, 2021Ĭopyright: © 2021 Forgacs et al. Huber, University of South Dakota, UNITED STATES (2021) Convergent antibody evolution and clonotype expansion following influenza virus vaccination. Collectively, this high-resolution antibody repertoire analysis demonstrated the impact evolution can have on BCRs in response to influenza virus vaccination, which can guide future universal influenza prophylactic approaches.Ĭitation: Forgacs D, Abreu RB, Sautto GA, Kirchenbaum GA, Drabek E, Williamson KS, et al. Binding profiles of one private and three public PBs confirmed they were all subtype-specific, cross-reactive hemagglutinin (HA) head-directed antibodies. Approximately 60% (10/17) of participants experienced convergent evolution, possessing public PBs that were elicited independently in multiple participants. In some participants, a single expanded clonotype accounted for ~22% of their PB BCR repertoire. A combination of Immune Repertoire Capture (IRC TM) technology and IgG sequencing was performed on ~7,800 plasmablast (PB) cells and preferential IgG heavy-light chain pairings were investigated. In this study, PBMCs were collected from 17 human participants vaccinated with the split-inactivated influenza virus vaccine during the 2016–2017 influenza season. Recent advances in high-throughput single cell sequencing have opened up new avenues into the investigation of B cell receptor (BCR) repertoires. ![]()
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